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pLV-mEGFP-hCTNNA1_Wild-type
(Plasmid #234551)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 234551 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    pLV[Exp]-CMV>mEGFP-_E-catenin EF1A(core)>Puro
  • Backbone manufacturer
    VectorBuilder
  • Vector type
    Lentiviral
  • Selectable markers
    Puromycin

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    NEB Stable
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    mEGFP:hCTNNA1_Wild-type
  • Alt name
    Alpha-E-catenin Wild-type
  • Species
    H. sapiens (human)
  • Mutation
    None
  • Entrez Gene
    CTNNA1 (a.k.a. CAP102, MDBS2, MDPT2)
  • Promoter RSV
  • Tag / Fusion Protein
    • mEGFP (N terminal on insert)

Cloning Information

  • Cloning method Gene Synthesis

Resource Information

  • A portion of this plasmid was derived from a plasmid made by
    Human gene

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.

Depositor Comments

Enhanced GFP contains A206K mutation to prevent GFP homodimerization

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pLV-mEGFP-hCTNNA1_Wild-type was a gift from Cara Gottardi (Addgene plasmid # 234551 ; http://n2t.net/addgene:234551 ; RRID:Addgene_234551)

  • For your References section:

    alpha-catenin middle- and actin-binding domain unfolding mutants differentially impact epithelial strength and sheet migration. Quinn JM, Wang Y, Wood M, Flozak AS, Le PM, Yemelyanov A, Oakes PW, Gottardi CJ. Mol Biol Cell. 2024 May 1;35(5):ar65. doi: 10.1091/mbc.E23-01-0036. Epub 2024 Mar 20. 10.1091/mbc.E23-01-0036 PubMed 38507238
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