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pLV-mEGFP-hCTNNA1_P-linker 4E
(Plasmid #234564)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 234564 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    pLV[Exp]-CMV>mEGFP-_E-catenin EF1A(core)>Puro
  • Backbone manufacturer
    VectorBuilder
  • Vector type
    Lentiviral
  • Selectable markers
    Puromycin

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    NEB Stable
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    mEGFP:hCTNNA1_P-linker 4E
  • Alt name
    Alpha-E-catenin P-linker phosho-mimic
  • Species
    H. sapiens (human)
  • Mutation
    S641E, S652E, S655E, T658E
  • Entrez Gene
    CTNNA1 (a.k.a. CAP102, MDBS2, MDPT2)
  • Promoter CMV
  • Tag / Fusion Protein
    • mEGFP (N terminal on insert)

Cloning Information

  • Cloning method Gene Synthesis

Resource Information

  • A portion of this plasmid was derived from a plasmid made by
    Human gene

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.

Depositor Comments

Enhanced GFP contains A206K mutation to prevent GFP homodimerization.

Please visit https://doi.org/10.1101/2024.09.06.611639 for bioRxiv preprint.

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pLV-mEGFP-hCTNNA1_P-linker 4E was a gift from Cara Gottardi (Addgene plasmid # 234564 ; http://n2t.net/addgene:234564 ; RRID:Addgene_234564)

  • For your References section:

    alpha -catenin phosphorylation is elevated during mitosis to resist apical rounding and epithelial barrier leak. Le PM, Quinn JM, Flozak AS, Steffeck AW, Huang CF, Gottardi CJ. bioRxiv [Preprint]. 2024 Sep 6:2024.09.06.611639. doi: 10.1101/2024.09.06.611639. 10.1101/2024.09.06.611639 PubMed 39282345
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