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Addgene

FLuc-C-NLuc
(Plasmid #234566)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 234566 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    pET21a (+)
  • Backbone manufacturer
    Novagen
  • Backbone size w/o insert (bp) 5409
  • Total vector size (bp) 7632
  • Vector type
    Bacterial Expression

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Growth instructions
    Depositing lab has also used Top10 E. coli for growth.
  • Copy number
    Low Copy

Gene/Insert

  • Gene/Insert name
    Firefly luciferase
  • Alt name
    FLuc-C-NLuc
  • Species
    Synthetic; P. pyralis
  • Insert Size (bp)
    2235
  • Mutation
    Codon optmization for expression in BL21DE3
  • GenBank ID
    M15077.1
  • Promoter T7
  • Tags / Fusion Proteins
    • T7 gene 10 leader peptide (N terminal on insert)
    • NanoLuciferase-thrombin site-10xHis (C terminal on insert)

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site BamH1 (unknown if destroyed)
  • 3′ cloning site Xho1 (unknown if destroyed)
  • 5′ sequencing primer T7 promoter primer #69348-3
  • 3′ sequencing primer T7 terminator primer #69337-3
    • (Common Sequencing Primers)

Resource Information

  • A portion of this plasmid was derived from a plasmid made by
    synthesis by Genscript

Terms and Licenses

Trademarks:
  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    FLuc-C-NLuc was a gift from James Inglese (Addgene plasmid # 234566 ; http://n2t.net/addgene:234566 ; RRID:Addgene_234566)

  • For your References section:

    A general assay platform to study protein pharmacology using ligand-dependent structural dynamics. Ciulla DA, Dranchak PK, Aitha M, van Neer RHP, Shah D, Tharakan R, Wilson KM, Wang Y, Braisted JC, Inglese J. Nat Commun. 2025 May 10;16(1):4342. doi: 10.1038/s41467-025-59658-6. 10.1038/s41467-025-59658-6 PubMed 40346061
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