pSLB026
(Plasmid
#234635)
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PurposepET-28a(+) based plasmid for expression of H16_A3514 from Cupriavidus necator H16, with N-terminal hexahistidine tag
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Depositing Lab
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Sequence Information
Ordering
Item | Catalog # | Description | Quantity | Price (USD) | |
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Plasmid | 234635 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 |
Backbone
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Vector backbonepET-28a(+)
- Total vector size (bp) 6966
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Vector typeBacterial Expression
Growth in Bacteria
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Bacterial Resistance(s)Kanamycin, 50 μg/mL
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Growth Temperature37°C
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Growth Strain(s)DH5alpha
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Copy numberHigh Copy
Gene/Insert
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Gene/Insert nameH16_A3514
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Entrez GeneH16_RS17530 (a.k.a. H16_RS17530, H16_A3514)
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Tag
/ Fusion Protein
- 6x-His (N terminal on insert)
Resource Information
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Supplemental Documents
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A portion of this plasmid was derived from a plasmid made byPlasmids were first synthesized by TWIST and were re-prepared in house.
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
pSLB026 was a gift from Gregg Beckham (Addgene plasmid # 234635 ; http://n2t.net/addgene:234635 ; RRID:Addgene_234635)