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Addgene

pET30a-GalK-USP-ET64
(Plasmid #234662)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 234662 Standard format: Plasmid sent in bacteria as agar stab 1 $89
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Backbone

  • Vector backbone
    pET30a
  • Vector type
    Bacterial Expression

Growth in Bacteria

  • Bacterial Resistance(s)
    Kanamycin, 50 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    High Copy

Gene/Insert 1

  • Gene/Insert name
    galactokinase
  • Alt name
    GalK
  • Species
    Streptococcus pneumoniae TIGR4

Gene/Insert 2

  • Gene/Insert name
    UDP-sugar pyrophosphorylase
  • Alt name
    AtUSP
  • Species
    A. thaliana (mustard weed)
  • Entrez Gene
    USP (a.k.a. AT5G52560, ATUSP, F6N7.4, F6N7_4, UDP-sugar pyrophosphorylase)
  • Tag / Fusion Protein
    • ELP (C terminal on insert)

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.

Depositor Comments

The depositor notes that the Addgene sequence, when compared to the depositor provided sequence, may contain the following discrepancies, which are not of concern for plasmid function:
443bp indel in insert.

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pET30a-GalK-USP-ET64 was a gift from Xianwei Liu (Addgene plasmid # 234662 ; http://n2t.net/addgene:234662 ; RRID:Addgene_234662)

  • For your References section:

    Protein Fusion of Biosynthetic Enzymes and a Thermo-Responsive Polypeptide Expedites Facile Access to Biocatalysts for Nucleotide Sugars. Wu X, Liu J, Yin X, Ma D, Zhang S, Liu X. Chembiochem. 2025 Jan 13:e202401005. doi: 10.1002/cbic.202401005. 10.1002/cbic.202401005 PubMed 39805738
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