Skip to main content
Addgene

Cyclin B1-Venus ND
(Plasmid #234673)

Print

Ordering

This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 234673 Standard format: Plasmid sent in bacteria as agar stab 1 $85
Add to Cart

Backbone

  • Vector backbone
    pVenus-N1
  • Backbone size w/o insert (bp) 4688
  • Total vector size (bp) 6008
  • Vector type
    Mammalian Expression
  • Selectable markers
    Neomycin (select with G418)

Growth in Bacteria

  • Bacterial Resistance(s)
    Kanamycin, 50 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    High Copy

Gene/Insert 1

  • Gene/Insert name
    Cyclin B1
  • Species
    H. sapiens (human)
  • Insert Size (bp)
    1941
  • GenBank ID
    BC006510.2
  • Entrez Gene
    CCNB1 (a.k.a. CCNB)
  • Promoter CMV
  • Tag / Fusion Protein
    • Venus (C terminal on insert)

Cloning Information for Gene/Insert 1

  • Cloning method Restriction Enzyme
  • 5′ cloning site HindIII (unknown if destroyed)
  • 3′ cloning site BamHI (unknown if destroyed)
  • 5′ sequencing primer CMV promoter
  • 3′ sequencing primer SV40 poly A reverse
    • (Common Sequencing Primers)

Gene/Insert 2

  • Gene/Insert name
    Cyclin B1
  • Species
    H. sapiens (human)
  • Mutation
    double mutation R42A and L45A
  • GenBank ID
    BC006510.2
  • Entrez Gene
    CCNB1 (a.k.a. CCNB)
  • Tag / Fusion Protein
    • Venus (C terminal on insert)

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.

Depositor Comments

the cyclin B1 was rendered stably upon R42A/L45A point mutations

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    Cyclin B1-Venus ND was a gift from Lienhard Schmitz (Addgene plasmid # 234673 ; http://n2t.net/addgene:234673 ; RRID:Addgene_234673)

  • For your References section:

    CDK1-mediated phosphorylation at H2B serine 6 is required for mitotic chromosome segregation. Seibert M, Kruger M, Watson NA, Sen O, Daum JR, Slotman JA, Braun T, Houtsmuller AB, Gorbsky GJ, Jacob R, Kracht M, Higgins JMG, Schmitz ML. J Cell Biol. 2019 Apr 1;218(4):1164-1181. doi: 10.1083/jcb.201806057. Epub 2019 Feb 14. 10.1083/jcb.201806057 PubMed 30765437
Related items: