pTcINDEX-GW
(Plasmid
#234713)
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Purpose(Empty Backbone) Used to express any CDS in T. cruzi in a tetracycline regulatable manner. Modified from Taylor et al. (2006) for easy cloning using Gateway recombination cassette.
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Depositing Lab
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Sequence Information
Ordering
Item | Catalog # | Description | Quantity | Price (USD) | |
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Plasmid | 234713 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 |
Backbone
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Vector backbonepTcINDEX
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Vector typeTrypanosomatid expression
Growth in Bacteria
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Bacterial Resistance(s)Chloramphenicol and Ampicillin, 25 & 100 μg/mL
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Growth Temperature37°C
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Growth Strain(s)ccdB Survival
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Copy numberHigh Copy
Gene/Insert
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Gene/Insert nameNone
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
Depositor Comments
5' Cloning Site: NruI (destroyed), 3' Cloning Site: NruI (destroyed).
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
pTcINDEX-GW was a gift from Esteban Serra (Addgene plasmid # 234713 ; http://n2t.net/addgene:234713 ; RRID:Addgene_234713) -
For your References section:
Construction of three new Gateway(R) expression plasmids for Trypanosoma cruzi. Alonso VL, Ritagliati C, Cribb P, Serra EC. Mem Inst Oswaldo Cruz. 2014 Dec;109(8):1081-5. doi: 10.1590/0074-0276140238. Epub 2014 Nov 21. 10.1590/0074-0276140238 PubMed 25424446