pTcTurboID-GW
(Plasmid
#234714)
-
Purpose(Empty Backbone) Used to express any CDS fused to 3xHA-TurboID (N-terminal) in T. cruzi. Tetracycline regulated. Modified from Alonso et al. (2014).
-
Depositing Lab
-
Publication
-
Sequence Information
Ordering
This material is available to academics and nonprofits only.
| Item | Catalog # | Description | Quantity | Price (USD) | |
|---|---|---|---|---|---|
| Plasmid | 234714 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 | |
Backbone
-
Vector backbonepTcTurboID-GW
-
Vector typeTrypanosomatid expression
-
Tag
/ Fusion Protein
- 3xHA (N terminal on backbone)
Growth in Bacteria
-
Bacterial Resistance(s)Chloramphenicol and Ampicillin, 25 & 100 μg/mL
-
Growth Temperature37°C
-
Growth Strain(s)ccdB Survival
-
Copy numberHigh Copy
Gene/Insert
-
Gene/Insert nameNone
-
Tag
/ Fusion Protein
- 3xHA (N terminal on backbone)
Terms and Licenses
-
Academic/Nonprofit Terms
-
Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
Depositor Comments
If you want to perform traditional cloning (instead of Gateway recombiantion), it can be achieved by replacing the GFP cassette from pTcTurboID-GW_GFP with the desired CDS using BamHI and EcoRV restriction enzymes. Also, any CDS can be cloned in frame to GFP using only EcoRV. 5' Cloning Site: ClaI (not destroyed), 3' Cloning Site: ClaI (not destroyed).
How to cite this plasmid
( Back to top)
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
-
For your Materials & Methods section:
pTcTurboID-GW was a gift from Esteban Serra (Addgene plasmid # 234714 ; http://n2t.net/addgene:234714 ; RRID:Addgene_234714)
