pEE082 FoMV
(Plasmid
#234811)
-
Purpose(Empty Backbone) To clone guide RNA (gRNA) along with its scaffold in to the Foxtail Mosaic Virus Vector (T-DNA)
-
Depositing Lab
-
Sequence Information
Ordering
Item | Catalog # | Description | Quantity | Price (USD) | |
---|---|---|---|---|---|
Plasmid | 234811 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 |
Backbone
-
Vector backboneUnspecified
-
Vector typePlant Expression
Growth in Bacteria
-
Bacterial Resistance(s)Kanamycin, 50 μg/mL
-
Growth Temperature37°C
-
Growth Strain(s)ccdB Survival
-
Copy numberHigh Copy
Gene/Insert
-
Gene/Insert nameNone
Resource Information
-
Supplemental Documents
Terms and Licenses
-
Academic/Nonprofit Terms
-
Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
Depositor Comments
the ccdB gene flanked on both sites by SapI for the insertion of target sequence.
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
-
For your Materials & Methods section:
pEE082 FoMV was a gift from Daniel Voytas (Addgene plasmid # 234811 ; http://n2t.net/addgene:234811 ; RRID:Addgene_234811) -
For your References section:
Rapid and efficient in planta genome editing in sorghum using foxtail mosaic virus-mediated sgRNA delivery. Baysal C, Kausch AP, Cody JP, Altpeter F, Voytas DF. Plant J. 2025 Jan;121(2):e17196. doi: 10.1111/tpj.17196. Epub 2024 Dec 11. 10.1111/tpj.17196 PubMed 39661735