Skip to main content
Addgene

pCB094 FoMV-sgLw
(Plasmid #234816)

Print

Ordering

This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 234816 Standard format: Plasmid sent in bacteria as agar stab 1 $85
Add to Cart

Backbone

  • Vector backbone
    Unspecified
  • Vector type
    Plant Expression

Growth in Bacteria

  • Bacterial Resistance(s)
    Kanamycin, 50 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    gRNA targeting SbLw
  • gRNA/shRNA sequence
    Unspecified
  • Species
    Foxtail Mosaic Virus

Cloning Information

Resource Information

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.

Depositor Comments

The forward oligo included SapI restriction sites, the duplicated coat protein promoter sequence, and 20-bp sgRNA sequences flanked by the 5' sequences of the sgRNA scaffold. A common reverse oligo (esgRNA in FoMV R) was used to amplify the 3' region of the sgRNA scaffold, including a SapI restriction site for subsequent cloning.

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pCB094 FoMV-sgLw was a gift from Daniel Voytas (Addgene plasmid # 234816 ; http://n2t.net/addgene:234816 ; RRID:Addgene_234816)

  • For your References section:

    Rapid and efficient in planta genome editing in sorghum using foxtail mosaic virus-mediated sgRNA delivery. Baysal C, Kausch AP, Cody JP, Altpeter F, Voytas DF. Plant J. 2025 Jan;121(2):e17196. doi: 10.1111/tpj.17196. Epub 2024 Dec 11. 10.1111/tpj.17196 PubMed 39661735
Related items: