pRS118
(Plasmid
#234878)
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Purpose(Empty Backbone) CRISPR-Cas9 plasmid for use in Candida albicans. Contains Cas9, NEUT5L integration site, and the sgRNA cloning site (SNR52 promoter)
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Depositing Lab
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Sequence Information
Ordering
This material is available to academics and nonprofits only.
| Item | Catalog # | Description | Quantity | Price (USD) | |
|---|---|---|---|---|---|
| Plasmid | 234878 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $89 | |
Backbone
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Vector backbonepRS118
- Backbone size (bp) 12462
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Vector typeYeast Expression, CRISPR, Synthetic Biology
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Selectable markersNourseothricin
Growth in Bacteria
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Bacterial Resistance(s)Ampicillin, 100 μg/mL
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Growth Temperature37°C
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Growth Strain(s)DH5alpha
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Copy numberHigh Copy
How to cite this plasmid
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These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
pRS118 was a gift from Rebecca Shapiro (Addgene plasmid # 234878 ; http://n2t.net/addgene:234878 ; RRID:Addgene_234878) -
For your References section:
Targeted loss of heterozygosity in Candida albicans using CRISPR-Cas9 reveals the functional impact of allelic variation. Després PC, Gervais NC, Fogal M, Rogers RKJ, Cuomo CA, Shapiro RS. Genetics. 2025 Oct 8;231(2):iyaf154. doi: 10.1093/genetics/iyaf154. 10.1093/genetics/iyaf154 PubMed 40794772
