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pLVXNeo_SPARC-mSc-SBP
(Plasmid #234885)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 234885 Standard format: Plasmid sent in bacteria as agar stab 1 $89
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Backbone

  • Vector backbone
    pLVXNeo
  • Backbone manufacturer
    Takara
  • Backbone size w/o insert (bp) 8317
  • Total vector size (bp) 10117
  • Vector type
    Mammalian Expression, Lentiviral
  • Selectable markers
    Neomycin (select with G418)

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    NEB Stable
  • Copy number
    Unknown

Gene/Insert

  • Gene/Insert name
    SPARC
  • Alt name
    SPARC
  • Species
    H. sapiens (human)
  • Insert Size (bp)
    1800
  • Mutation
    N/A
  • GenBank ID
    NM_003118.4
  • Entrez Gene
    SPARC (a.k.a. BM-40, OI17, ON, ONT)
  • Promoter CMV
  • Tags / Fusion Proteins
    • SBP (C terminal on insert)
    • mScarleti (C terminal on insert)

Cloning Information

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pLVXNeo_SPARC-mSc-SBP was a gift from Nicola Stevenson (Addgene plasmid # 234885 ; http://n2t.net/addgene:234885 ; RRID:Addgene_234885)

  • For your References section:

    Multiple golgins are required to support extracellular matrix secretion, modification, and assembly. Thompson G, Hoyle A, Lewis PA, Prada-Sanchez ME, Swift J, Heesom K, Lowe M, Stephens D, Stevenson NL. J Cell Biol. 2025 Oct 6;224(10):e202411167. doi: 10.1083/jcb.202411167. Epub 2025 Aug 18. 10.1083/jcb.202411167 PubMed 40824304
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