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CAG-NGN3-linker-3xFLAG-Dendra2-HA
(Plasmid #234887)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 234887 Standard format: Plasmid sent in bacteria as agar stab 1 $89
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Backbone

  • Vector backbone
    pRP[Exp]
  • Vector type
    Mammalian Expression
  • Selectable markers
    Puromycin

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    NGN3-3xFLAG-Dendra2-HA
  • Alt name
    NEUROG3
  • Species
    H. sapiens (human)
  • Entrez Gene
    NEUROG3 (a.k.a. Atoh5, Math4B, NGN-3, bHLHa7, ngn3)
  • Promoter CAG
  • Tag / Fusion Protein
    • 3xFLAG, Dendra2 and HA (C terminal on insert)

Cloning Information

Resource Information

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.

Depositor Comments

Please visit https://doi.org/10.1101/2024.01.10.574974 for bioRxiv preprint.

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    CAG-NGN3-linker-3xFLAG-Dendra2-HA was a gift from Nancy Papalopulu (Addgene plasmid # 234887 ; http://n2t.net/addgene:234887 ; RRID:Addgene_234887)

  • For your References section:

    NGN3 oscillatory expression controls the timing of human pancreatic endocrine differentiation. Miller A, Biga V, Rowntree A, Chhatriwala M, Woods F, Noble B, Marinopoulou E, Kamath A, Vallier L, Papalopulu N. Dev Cell. 2025 Sep 22;60(18):2518-2532.e9. doi: 10.1016/j.devcel.2025.06.004. Epub 2025 Jun 25. 10.1016/j.devcel.2025.06.004 PubMed 40570854
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