pAAV-pCAG-FRT(F3F5)-ChRmine3.3-p2A-oScarlet,rev.-WPRE
(Plasmid
#234895)
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PurposeCAG-driven, Flp-dependent ChRmine for optogenetic excitation of neurons with an oScarlet tag
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Depositing Lab
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Sequence Information
Ordering
Item | Catalog # | Description | Quantity | Price (USD) | |
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Plasmid | 234895 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 |
Backbone
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Vector backbonepAAV
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Vector typeAAV
Growth in Bacteria
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Bacterial Resistance(s)Ampicillin, 100 μg/mL
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Growth Temperature37°C
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Growth Strain(s)NEB Stable
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Copy numberUnknown
Gene/Insert
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Gene/Insert nameFRT(F3F5)-ChRmine3.3-p2A-oScarlet
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SpeciesSynthetic
Cloning Information
- Cloning method Gibson Cloning
Resource Information
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Supplemental Documents
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
pAAV-pCAG-FRT(F3F5)-ChRmine3.3-p2A-oScarlet,rev.-WPRE was a gift from Silvia Arber (Addgene plasmid # 234895 ; http://n2t.net/addgene:234895 ; RRID:Addgene_234895) -
For your References section:
Dynamic basal ganglia output signals license and suppress forelimb movements. Falasconi A, Kanodia H, Arber S. Nature. 2025 May 28. doi: 10.1038/s41586-025-09066-z. 10.1038/s41586-025-09066-z PubMed 40437098