pVS-GhSP
(Plasmid
#234899)
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PurposeThis all-in-one vector is used to specifically silence the GhSP gene via virus-induced gene silencing (VIGS).
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Depositing Lab
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Sequence Information
Ordering
Item | Catalog # | Description | Quantity | Price (USD) | |
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Plasmid | 234899 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 |
Backbone
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Vector backbonepJRT.Agro.CLCrVA.008
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Backbone manufacturerTuttle et al Plant Methods. 2012 Aug 1;8(1):27. doi: 10.1186/1746-4811-8-27.
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Vector typePlant Expression
Growth in Bacteria
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Bacterial Resistance(s)Kanamycin, 50 μg/mL
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Growth Temperature37°C
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Growth Strain(s)DH5alpha
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Copy numberHigh Copy
Gene/Insert
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Gene/Insert nameVIGS fragement of GhSP gene
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SpeciesG. hirsutum (cotton)
Cloning Information
- Cloning method Other
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
pVS-GhSP was a gift from Guoli Song (Addgene plasmid # 234899 ; http://n2t.net/addgene:234899 ; RRID:Addgene_234899) -
For your References section:
An all-in-one plant virus-based vector toolkit for streamlined gene silencing, overexpression, and genome editing. Hao Y, Yuan Z, Li Y, Zuo D, Cheng H, Wang Q, Zhang Y, Lv L, Liu J, Song G. Plant Commun. 2025 May 19:101379. doi: 10.1016/j.xplc.2025.101379. 10.1016/j.xplc.2025.101379 PubMed 40394900