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Addgene

ABE8e SpG Puro
(Plasmid #235044)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 235044 Standard format: Plasmid sent in bacteria as agar stab 1 $89
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Backbone

  • Vector backbone
    pRDA_479 (Addgene Plasmid #179099)
  • Backbone manufacturer
    John Doench, David Root
  • Vector type
    Lentiviral, CRISPR
  • Selectable markers
    Puromycin

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    NEB Stable
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    ABE8e, nCas9
  • Species
    H. sapiens (human)
  • Promoter EF1a core
  • Tag / Fusion Protein
    • P2A-PuroR (C terminal on insert)

Cloning Information

  • Cloning method Other

Resource Information

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.

Depositor Comments

pRDA_479 (Plasmid #179099) without U6 sgRNA expression

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    ABE8e SpG Puro was a gift from Justin Pritchard (Addgene plasmid # 235044 ; http://n2t.net/addgene:235044 ; RRID:Addgene_235044)

  • For your References section:

    A side-by-side comparison of variant function measurements using deep mutational scanning and base editing. Sokirniy I, Inam H, Tomaszkiewicz M, Reynolds J, McCandlish D, Pritchard J. Nucleic Acids Res. 2025 Jul 19;53(14):gkaf738. doi: 10.1093/nar/gkaf738. 10.1093/nar/gkaf738 PubMed 40744495
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