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nSpG UGI Puro
(Plasmid #235046)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 235046 Standard format: Plasmid sent in bacteria as agar stab 1 $89
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Backbone

  • Vector backbone
    pRDA_256 (Addgene Plasmid #158581)
  • Backbone manufacturer
    John Doench, David Root
  • Vector type
    Lentiviral, CRISPR
  • Selectable markers
    Puromycin

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    NEB Stable
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    nCas9
  • Species
    Synthetic
  • Promoter EF1a core
  • Tag / Fusion Protein
    • P2A-PuroR (C terminal on insert)

Cloning Information

  • Cloning method Other

Resource Information

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry

Trademarks:

  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    nSpG UGI Puro was a gift from Justin Pritchard (Addgene plasmid # 235046 ; http://n2t.net/addgene:235046 ; RRID:Addgene_235046)

  • For your References section:

    A side-by-side comparison of variant function measurements using deep mutational scanning and base editing. Sokirniy I, Inam H, Tomaszkiewicz M, Reynolds J, McCandlish D, Pritchard J. Nucleic Acids Res. 2025 Jul 19;53(14):gkaf738. doi: 10.1093/nar/gkaf738. 10.1093/nar/gkaf738 PubMed 40744495
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