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Addgene

Tad-CABE2.5
(Plasmid #235054)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 235054 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    pCMV with a BR322 origin
  • Backbone size w/o insert (bp) 3402
  • Vector type
    Mammalian Expression, CRISPR

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    Tad-CABE2.5-SpCas9 D10A
  • Species
    Synthetic
  • Insert Size (bp)
    5373
  • Tags / Fusion Proteins
    • bpNLS (N terminal on insert)
    • 2xUGI, SV40NLS (C terminal on insert)

Cloning Information

  • Cloning method Golden Gate

Resource Information

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    Tad-CABE2.5 was a gift from Weixin Tang (Addgene plasmid # 235054 ; http://n2t.net/addgene:235054 ; RRID:Addgene_235054)

  • For your References section:

    High-precision cytosine base editors by evolving nucleic-acid-recognition hotspots in deaminase. Wu Y, Xiao YL, Tang W. Nat Biotechnol. 2025 Jul 7:10.1038/s41587-025-02678-w. doi: 10.1038/s41587-025-02678-w. 10.1038/s41587-025-02678-w PubMed 40624252
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