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BFP-GFP-bidirectional MS2 splicing reporter FRG1-intron
(Plasmid #235087)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 235087 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    pMF4-AAV
  • Vector type
    Mammalian Expression, AAV

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    NEB Stable
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    BFP-GFP-bidirectional MS2 splicing reporter FRG1-intron
  • Species
    H. sapiens (human)
  • Entrez Gene
    FRG1 (a.k.a. FRG1A, FSG1)
  • Promoter custom bidirectional

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site Unknown (unknown if destroyed)
  • 3′ cloning site Unknown (unknown if destroyed)

Resource Information

Terms and Licenses

Trademarks:
  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    BFP-GFP-bidirectional MS2 splicing reporter FRG1-intron was a gift from Mitchell Guttman (Addgene plasmid # 235087 ; http://n2t.net/addgene:235087 ; RRID:Addgene_235087)

  • For your References section:

    Genome organization around nuclear speckles drives mRNA splicing efficiency. Bhat P, Chow A, Emert B, Ettlin O, Quinodoz SA, Strehle M, Takei Y, Burr A, Goronzy IN, Chen AW, Huang W, Ferrer JLM, Soehalim E, Goh ST, Chari T, Sullivan DK, Blanco MR, Guttman M. Nature. 2024 May;629(8014):1165-1173. doi: 10.1038/s41586-024-07429-6. Epub 2024 May 8. 10.1038/s41586-024-07429-6 PubMed 38720076
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