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VN1272 pB2Hws_v28_Ddcm_wkKanR_TIR51_pLWeakRBS_TIR371_cI-2GG+rpoA-IP3
(Plasmid #235120)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 235120 Standard format: Plasmid sent in bacteria as agar stab 1 $89
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Backbone

  • Vector backbone
    pBR
  • Backbone size (bp) 5094
  • Vector type
    Bacterial Expression, Synthetic Biology ; Quantitative bacterial two-hybrid (qB2H)
  • Tag / Fusion Protein
    • rpoA (N terminal on insert)

Growth in Bacteria

  • Bacterial Resistance(s)
    Chloramphenicol, 25 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    SB39 (Addgene #235121)
  • Growth instructions
    Resistant to kanamycin (dependent on the interaction strength with partner, VN515-VN519)
  • Copy number
    Low Copy

Gene/Insert

  • Gene/Insert name
    None
  • Tag / Fusion Protein
    • rpoA (N terminal on insert)

Resource Information

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.

Depositor Comments

BsaI sites for Golden Gate cloning. Contains IP3.

Please note that this plasmid may require a unique bacterial strain, so make sure to confirm that you can also obtain the appropriate growth strain. Please contact us at [email protected] or contact our distributors if you have any questions.

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    VN1272 pB2Hws_v28_Ddcm_wkKanR_TIR51_pLWeakRBS_TIR371_cI-2GG+rpoA-IP3 was a gift from Oscar Ramos (Addgene plasmid # 235120 ; http://n2t.net/addgene:235120 ; RRID:Addgene_235120)
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