pLD1112 TRE-mCherry-SKL-22Xm6A MS2
(Plasmid
#235128)
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PurposemCherry reporter plasmid for live cell visualization of RNA m6A modification
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Depositing Lab
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Sequence Information
Ordering
Item | Catalog # | Description | Quantity | Price (USD) | |
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Plasmid | 235128 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $89 |
Backbone
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Vector backbonepCW57.1
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Vector typeMammalian Expression, Lentiviral
Growth in Bacteria
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Bacterial Resistance(s)Ampicillin, 100 μg/mL
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Growth Temperature30°C
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Growth Strain(s)NEB Stable
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Copy numberHigh Copy
Gene/Insert
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Gene/Insert nameTRE-mCherry-SKL-22Xm6A MS2
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SpeciesH. sapiens (human)
Cloning Information
- Cloning method Restriction Enzyme
- 5′ cloning site Unknown (unknown if destroyed)
- 3′ cloning site Unknown (unknown if destroyed)
Resource Information
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Supplemental Documents
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
pLD1112 TRE-mCherry-SKL-22Xm6A MS2 was a gift from Qin Peng (Addgene plasmid # 235128 ; http://n2t.net/addgene:235128 ; RRID:Addgene_235128) -
For your References section:
Decoding the interplay between m(6)A modification and stress granule stability by live-cell imaging. Li Q, Liu J, Guo L, Zhang Y, Chen Y, Liu H, Cheng H, Deng L, Qiu J, Zhang K, Goh WSS, Wang Y, Peng Q. Sci Adv. 2024 Nov 15;10(46):eadp5689. doi: 10.1126/sciadv.adp5689. Epub 2024 Nov 15. 10.1126/sciadv.adp5689 PubMed 39546601