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pB–DualPE
(Plasmid #235161)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 235161 Standard format: Plasmid sent in bacteria as agar stab 1 $89
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Backbone

  • Vector backbone
    pBUE411
  • Vector type
    CRISPR
  • Selectable markers
    Basta

Growth in Bacteria

  • Bacterial Resistance(s)
    Kanamycin, 50 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    Unknown

Gene/Insert 1

  • Gene/Insert name
    Csy4-P2A-Nls-nCas9-NC-nls-MLV-Nls
  • Species
    Synthetic; SpCas9 is from Streptococcus pyogenes; MMLV_RT is from the Moloney murine leukemia virus
  • Insert Size (bp)
    6807
  • Mutation
    Detailed in manuscript

Cloning Information for Gene/Insert 1

Gene/Insert 2

  • Gene/Insert name
    CmYLCV-epegRNA-CaMV poly(A) signal
  • Species
    Synthetic
  • Insert Size (bp)
    841
  • Mutation
    Detailed in manuscript

Cloning Information for Gene/Insert 2

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pB–DualPE was a gift from Zong Yuan (Addgene plasmid # 235161 ; http://n2t.net/addgene:235161 ; RRID:Addgene_235161)

  • For your References section:

    Precise deletion, replacement and inversion of large DNA fragments in plants using dual prime editing. Zhao Y, Huang Z, Zhou X, Teng W, Liu Z, Wang W, Tang S, Liu Y, Liu J, Wang W, Chai L, Zhang N, Guo W, Liu J, Ni Z, Sun Q, Wang Y, Zong Y. Nat Plants. 2025 Feb;11(2):191-205. doi: 10.1038/s41477-024-01898-3. Epub 2025 Jan 13. 10.1038/s41477-024-01898-3 PubMed 39805934
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