pSIBR2.0-818-Cas12a-NT
(Plasmid
#235168)
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PurposeExpresses the SIBR2.0-818-Cas12a system and a NT crRNA
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Depositing Lab
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Sequence Information
Ordering
Item | Catalog # | Description | Quantity | Price (USD) | |
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Plasmid | 235168 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 |
Backbone
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Vector backbonepBBR1
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Vector typeBacterial Expression
Growth in Bacteria
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Bacterial Resistance(s)Kanamycin, 50 μg/mL
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Growth Temperature37°C
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Growth Strain(s)DH5alpha
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Copy numberUnknown
Gene/Insert
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Gene/Insert nameSIBR2.0-818-Cas12a
Resource Information
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Supplemental Documents
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
Depositor Comments
Please visit https://doi.org/10.1101/2024.11.24.625072 for bioRxiv preprint.
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
pSIBR2.0-818-Cas12a-NT was a gift from Constantinos Patinios (Addgene plasmid # 235168 ; http://n2t.net/addgene:235168 ; RRID:Addgene_235168) -
For your References section:
Streamlined and efficient genome editing in Cupriavidus necator H16 using an optimised SIBR-Cas system. Della Valle S, Orsi E, Creutzburg SCA, Jansen LFM, Pentari E, Beisel CL, Steel H, Nikel PI, Staals RHJ, Claassens NJ, van der Oost J, Huang WE, Patinios C. bioRxiv 2024.11.24.625072; doi: 10.1101/2024.11.24.625072 10.1101/2024.11.24.625072