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Addgene

pSIBR2.0-818-Cas12a-NT
(Plasmid #235168)

Ordering

This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 235168 Standard format: Plasmid sent in bacteria as agar stab 1 $85

Backbone

  • Vector backbone
    pBBR1
  • Vector type
    Bacterial Expression

Growth in Bacteria

  • Bacterial Resistance(s)
    Kanamycin, 50 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    Unknown

Gene/Insert

  • Gene/Insert name
    SIBR2.0-818-Cas12a

Resource Information

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.

Depositor Comments

Please visit https://doi.org/10.1101/2024.11.24.625072 for bioRxiv preprint.

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pSIBR2.0-818-Cas12a-NT was a gift from Constantinos Patinios (Addgene plasmid # 235168 ; http://n2t.net/addgene:235168 ; RRID:Addgene_235168)
  • For your References section:

    Streamlined and efficient genome editing in Cupriavidus necator H16 using an optimised SIBR-Cas system. Della Valle S, Orsi E, Creutzburg SCA, Jansen LFM, Pentari E, Beisel CL, Steel H, Nikel PI, Staals RHJ, Claassens NJ, van der Oost J, Huang WE, Patinios C. bioRxiv 2024.11.24.625072; doi: 10.1101/2024.11.24.625072 10.1101/2024.11.24.625072