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Addgene

FLAG-GRP78-NLS-Mut
(Plasmid #235206)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 235206 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    pcDNA3.1
  • Backbone size w/o insert (bp) 5500
  • Total vector size (bp) 9599
  • Vector type
    Mammalian Expression
  • Selectable markers
    Neomycin (select with G418)

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    Unknown

Gene/Insert

  • Gene/Insert name
    78-kDa glucose-regulated protein
  • Alt name
    GRP78
  • Alt name
    BiP
  • Alt name
    HSPA5
  • Species
    H. sapiens (human)
  • Insert Size (bp)
    4200
  • Mutation
    Mutate the NLS sequence
  • GenBank ID
    NM_005347.5
  • Entrez Gene
    HSPA5 (a.k.a. BIP, GRP78, HEL-S-89n)
  • Tag / Fusion Protein
    • FLAG (N terminal on insert)

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    FLAG-GRP78-NLS-Mut was a gift from Amy Shiu Lee (Addgene plasmid # 235206 ; http://n2t.net/addgene:235206 ; RRID:Addgene_235206)

  • For your References section:

    ER chaperone GRP78/BiP translocates to the nucleus under stress and acts as a transcriptional regulator. Liu Z, Liu G, Ha DP, Wang J, Xiong M, Lee AS. Proc Natl Acad Sci U S A. 2023 Aug;120(31):e2303448120. doi: 10.1073/pnas.2303448120. Epub 2023 Jul 24. 10.1073/pnas.2303448120 PubMed 37487081
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