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Addgene

FLAG-GRP78-R197H
(Plasmid #235208)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 235208 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    pcDNA3.1
  • Backbone size w/o insert (bp) 5500
  • Total vector size (bp) 9599
  • Vector type
    Mammalian Expression
  • Selectable markers
    Neomycin (select with G418)

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    Unknown

Gene/Insert

  • Gene/Insert name
    78-kDa glucose-regulated protein
  • Alt name
    GRP78
  • Alt name
    BiP
  • Alt name
    HSPA5
  • Species
    H. sapiens (human)
  • Insert Size (bp)
    4200
  • Mutation
    Change Arginine 197 to Histidine
  • GenBank ID
    NM_005347.5
  • Entrez Gene
    HSPA5 (a.k.a. BIP, GRP78, HEL-S-89n)
  • Tag / Fusion Protein
    • FLAG (N terminal on insert)

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    FLAG-GRP78-R197H was a gift from Amy Shiu Lee (Addgene plasmid # 235208 ; http://n2t.net/addgene:235208 ; RRID:Addgene_235208)

  • For your References section:

    Characterization and mechanism of stress-induced translocation of 78-kilodalton glucose-regulated protein (GRP78) to the cell surface. Tsai YL, Zhang Y, Tseng CC, Stanciauskas R, Pinaud F, Lee AS. J Biol Chem. 2015 Mar 27;290(13):8049-64. doi: 10.1074/jbc.M114.618736. Epub 2015 Feb 11. 10.1074/jbc.M114.618736 PubMed 25673690
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