pAAV-hsyn1-iCre-P2A-mRuby3
(Plasmid #235248)

• Purpose: AAV-mediated expression of iCre and mRuby3 in mammalian cells
• Depositing Lab: Takeshi Imai
• Publication: Egashira et al Sci Adv. 2026 Jan 16;12(3):eadw8458. doi: 10.1126/sciadv.adw8458. Epub 2026 Jan 14.

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Backbone

• Vector backbone: pAAV
• Backbone size w/o insert (bp): 4569
• Total vector size (bp): 6405
• Vector type: Mammalian Expression, AAV, Cre/Lox

Growth in Bacteria

• Bacterial Resistance(s): Ampicillin, 100 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): NEB Stable
• Copy number: High Copy

Gene/Insert 1

• Gene/Insert name: iCre
• Species: Synthetic
• Insert Size (bp): 1059
• GenBank ID: AY056050
• Promoter: hsyn1

Cloning Information for Gene/Insert 1

• Cloning method: Gibson Cloning
• 5′ sequencing primer: gcactgccagcttcagcaccgcgga

Gene/Insert 2

• Gene/Insert name: mRuby3
• Species: Synthetic
• Insert Size (bp): 711
• GenBank ID: KX987299
• Promoter: hsyn1

Cloning Information for Gene/Insert 2

• Cloning method: Gibson Cloning
• 5′ sequencing primer: GGATCTCTGTGTCTGGTGTGGCTGA

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
• Industry Terms:
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