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Addgene

U6-miR.FF4
(Plasmid #235256)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 235256 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    pShip.USF (upstream forward)
  • Backbone manufacturer
    Galloway lab
  • Backbone size w/o insert (bp) 2046
  • Vector type
    Mammalian Expression, Synthetic Biology

Growth in Bacteria

  • Bacterial Resistance(s)
    Kanamycin, 50 μg/mL
  • Growth Temperature
    30°C
  • Growth Strain(s)
    NEB Stable
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    FF4 hairpin
  • Alt name
    miR-FF4
  • Alt name
    FF4
  • gRNA/shRNA sequence
    gCTTGAAGTCTTTAATTAAATAGTGAAGCCACAGATGTATTTAATTAAAGACTTCAAGCGGT
  • Species
    Synthetic
  • Insert Size (bp)
    62
  • Promoter U6

Cloning Information

  • Cloning method Golden Gate
  • 5′ sequencing primer gcgtttctacaaactcttcc
  • 3′ sequencing primer ATTL2 (CATCAGAGATTTTGAGACAC)
    • (Common Sequencing Primers)

Resource Information

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.

Depositor Comments

Alt name: pKG2600 ; Please visit https://doi.org/10.1101/2024.06.25.600629 for bioRxiv preprint.

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    U6-miR.FF4 was a gift from Kate Galloway (Addgene plasmid # 235256 ; http://n2t.net/addgene:235256 ; RRID:Addgene_235256)

  • For your References section:

    Model-guided design of microRNA-based gene circuits supports precise dosage of transgenic cargoes into diverse primary cells. Love KS, Johnstone CP, Peterman EL, Gaglione S, Birnbaum ME, Galloway KE. Cell Syst. 2025 Apr 22:101269. doi: 10.1016/j.cels.2025.101269. 10.1016/j.cels.2025.101269 PubMed 40300600
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