pLentiX1-[TRE3G-mRuby2-TS-FF4x1-bGH]-EFS-rtTA-T2A-mGL-P2A-PuroR-WPRE Sequences (3)

Addgene Sequences:

Full (1)

Depositing Scientist Sequences:

Partial (2)

Full Sequences from Addgene (1)

Based on next-generation sequencing (NGS) results where indicated (Addgene NGS Result), or assembled from reference sequences and/or Sanger results (Addgene Assembled Sequence).

Analyze Sequence

GenBank

SnapGene

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> Addgene NGS Result CGCTGATATAgggcccggtacctttaagaccaatgacttacaaggcagctgtagatcttagccacttttt aaaagaaaaggggggactggaagggctagctcactcccaacgaagacaagatctgctttttgcttgtact gggtctctctggttagaccagatctgagcctgggagctctctggctaactagggaacccactgcttaagc ctcaataaagcttgccttgagtgcttcaagtagtgtgtgcccgtctgttgtgtgactctggtaactagag atccctcagacccttttagtcagtgtggaaaatctctagcagtagtagttcatgtcatcttattattcag tatttataacttgcaaagaaatgaatatcagagagtgagaggaacttgtttattgcagcttataatggtt acaaataaagcaatagcatcacaaatttcacaaataaagcatttttttcactgcattctagttgtggttt gtccaaactcatcaatgtatcttatcatgtctggctctagctatcccgcccctaactccgcccatcccgc ccctaactccgcccagttccgcccattctccgccccatggctgactaattttttttatttatgcagaggc cgaggccgcctcggcctctgagctattccagaagtagtgaggaggcttttttggaggcctagggacgtac ccaattcgccctatagtgagtcgtattacgcgcgctcactggccgtcgttttacaacgtcgtgactggga aaaccctggcgttacccaacttaatcgccttgcagcacatccccctttcgccagctggcgtaatagcgaa gaggcccgcaccgatcgcccttcccaacagttgcgcagcctgaatggcgaatgggacgcgccctgtagcg gcgcattaagcgcggcgggtgtggtggttacgcgcagcgtgaccgctacacttgccagcgccctagcgcc cgctcctttcgctttcttcccttcctttctcgccacgttcgccggctttccccgtcaagctctaaatcgg gggctccctttagggttccgatttagtgctttacggcacctcgaccccaaaaaacttgattagggtgatg gttcacgtagtgggccatcgccctgatagacggtttttcgccctttgacgttggagtccacgttctttaa tagtggactcttgttccaaactggaacaacactcaaccctatctcggtctattcttttgatttataaggg attttgccgatttcggcctattggttaaaaaatgagctgatttaacaaaaatttaacgcgaattttaaca aaatattaacgcttacaatttaggtggcacttttcggggaaatgtgcgcggaacccctatttgtttattt ttctaaatacattcaaatatgtatccgctcatgagacaataaccctgataaatgcttcaataatattgaa aaaggaagagtatgagtattcaacatttccgtgtcgcccttattcccttttttgcggcattttgccttcc tgtttttgctcacccagaaacgctggtgaaagtaaaagatgctgaagatcagttgggtgcacgagtgggt tacatcgaactggatctcaacagcggtaagatccttgagagttttcgccccgaagaacgttttccaatga tgagcacttttaaagttctgctatgtggcgcggtattatcccgtattgacgccgggcaagagcaactcgg tcgccgcatacactattctcagaatgacttggttgagtactcaccagtcacagaaaagcatcttacggat ggcatgacagtaagagaattatgcagtgctgccataaccatgagtgataacactgcggccaacttacttc tgacaacgatcggaggaccgaaggagctaaccgcttttttgcacaacatgggggatcatgtaactcgcct tgatcgttgggaaccggagctgaatgaagccataccaaacgacgagcgtgacaccacgatgcctgtagca atggcaacaacgttgcgcaaactattaactggcgaactacttactctagcttcccggcaacaattaatag actggatggaggcggataaagttgcaggaccacttctgcgctcggcccttccggctggctggtttattgc tgataaatctggagccggtgagcgtgggtctcgcggtatcattgcagcactggggccagatggtaagccc tcccgtatcgtagttatctacacgacggggagtcaggcaactatggatgaacgaaatagacagatcgctg agataggtgcctcactgattaagcattggtaactgtcagaccaagtttactcatatatactttagattga tttaaaacttcatttttaatttaaaaggatctaggtgaagatcctttttgataatctcatgaccaaaatc ccttaacgtgagttttcgttccactgagcgtcagaccccgtagaaaagatcaaaggatcttcttgagatc ctttttttctgcgcgtaatctgctgcttgcaaacaaaaaaaccaccgctaccagcggtggtttgtttgcc ggatcaagagctaccaactctttttccgaaggtaactggcttcagcagagcgcagataccaaatactgtt cttctagtgtagccgtagttaggccaccacttcaagaactctgtagcaccgcctacatacctcgctctgc taatcctgttaccagtggctgctgccagtggcgataagtcgtgtcttaccgggttggactcaagacgata gttaccggataaggcgcagcggtcgggctgaacggggggttcgtgcacacagcccagcttggagcgaacg acctacaccgaactgagatacctacagcgtgagctatgagaaagcgccacgcttcccgaagggagaaagg cggacaggtatccggtaagcggcagggtcggaacaggagagcgcacgagggagcttccagggggaaacgc ctggtatctttatagtcctgtcgggtttcgccacctctgacttgagcgtcgatttttgtgatgctcgtca ggggggcggagcctatggaaaaacgccagcaacgcggcctttttacggttcctggccttttgctggcctt ttgctcacatgttctttcctgcgttatcccctgattctgtggataaccgtattaccgcctttgagtgagc tgataccgctcgccgcagccgaacgaccgagcgcagcgagtcagtgagcgaggaagcggaagagcgccca atacgcaaaccgcctctccccgcgcgttggccgattcattaatgcagctggcacgacaggtttcccgact ggaaagcgggcagtgagcgcaacgcaattaatgtgagttagctcactcattaggcaccccaggctttaca ctttatgcttccggctcgtatgttgtgtggaattgtgagcggataacaatttcacacaggaaacagctat gaccatgattacgccaagcgcgcaattaaccctcactaaagggaacaaaagctggagctgcaagcttaat gtagtcttatgcaatactcttgtagtcttgcaacatggtaacgatgagttagcaacatgccttacaagga gagaaaaagcaccgtgcatgccgattggtggaagtaaggtggtacgatcgtgccttattaggaaggcaac agacgggtctgacatggattggacgaaccactgaattgccgcattgcagagatattgtatttaagtgcct agctcgatacataaacgggtctctctggttagaccagatctgagcctgggagctctctggctaactaggg aacccactgcttaagcctcaataaagcttgccttgagtgcttcaagtagtgtgtgcccgtctgttgtgtg actctggtaactagagatccctcagacccttttagtcagtgtggaaaatctctagcagtggcgcccgaac agggacttgaaagcgaaagggaaaccagaggagctctctcgacgcaggactcggcttgctgaagcgcgca cggcaagaggcgaggggcggcgactggtgagtacgccaaaaattttgactagcggaggctagaaggagag agatgggtgcgagagcgtcagtattaagcgggggagaattagatcgcgatgggaaaaaattcggttaagg ccagggggaaagaaaaaatataaattaaaacatatagtatgggcaagcagggagctagaacgattcgcag ttaatcctggcctgttagaaacatcagaaggctgtagacaaatactgggacagctacaaccatcccttca gacaggatcagaagaacttagatcattatataatacagtagcaaccctctattgtgtgcatcaaaggata gagataaaagacaccaaggaagctttagacaagatagaggaagagcaaaacaaaagtaagaccaccgcac agcaagcggccgctgatcttcagacctggaggaggagatatgagggacaattggagaagtgaattatata aatataaagtagtaaaaattgaaccattaggagtagcacccaccaaggcaaagagaagagtggtgcagag agaaaaaagagcagtgggaataggagctttgttccttgggttcttgggagcagcaggaagcactatgggc gcagcgtcaatgacgctgacggtacaggccagacaattattgtctggtatagtgcagcagcagaacaatt tgctgagggctattgaggcgcaacagcatctgttgcaactcacagtctggggcatcaagcagctccaggc aagaatcctggctgtggaaagatacctaaaggatcaacagctcctggggatttggggttgctctggaaaa ctcatttgcaccactgctgtgccttggaatgctagttggagtaataaatctctggaacagatttggaatc acacgacctggatggagtgggacagagaaattaacaattacacaagcttaatacactccttaattgaaga atcgcaaaaccagcaagaaaagaatgaacaagaattattggaattagataaatgggcaagtttgtggaat tggtttaacataacaaattggctgtggtatataaaattattcataatgatagtaggaggcttggtaggtt taagaatagtttttgctgtactttctatagtgaatagagttaggcagggatattcaccattatcgtttca gacccacctcccaaccccgaggggacccgacaggcccgaaggaatagaagaagaaggtggagagagagac agagacagatccattcgattagtgaacggatctcgacggtatcggttaacttttaaaagaaaagggggga ttggggggtacagtgcaggggaaagaatagtagacataatagcaacagacatacaaactaaagaattaca aaaacaaattacaaaattcaaaattttatcgtccagtgtggtggcTACTagcgCCATAGAGCCCACCGCA TCcccagcatgcctgctattgtcttcccaatcctcccccttgctgtcctgccccaccccaccccccagaa tagaatgacacctactcagacaatgcgatgcaatttcctcattttattaggaaaggacagtgggagtggc accttccagggtcaaggaaggcacgggggaggggcaaacaacagatggctggCAACTAGAAGGCACAGGT GCAACTCCTCCTAGgtttaattaaagacttcaagcggCCACAagctTgttgTTACTTGTACAGCTCGTCC atcccaccaccaagcccggcgaacttggcaactgcgtgttcgcgttgtactacgaacatttcattgtcac tttcctctaacctttccaggcggtgatcaacggcatggataccgggcatcttgatgttcccgacggtctt ttttgacctgtaagttgttacgaaagagcaagacagatggccaccaccatcaactttcagtgccatatga gtgtatcccctcagaccaccatctgctggatacatcatctctgtattaggctcccaacccttggtcttct tctgcatcacgggaccattggagggaaagtttacccctctgacttggacgtggtaaacgagacagccatc ctcaaggctggtgtcctgcatgacggtgacgactccaccatcttcgtatctcgtaactctttcccaagta aaaccctcaggaaaggactgtttaaagaaatcaggaatgcctttcgggtacttgataaaagtacggctgc catacatgaacgacgtggcaagaatgtcaaaggcaaatggcaggggtcctccctcgatgactttgatcct catggtttgagttcccatgtacggattgccttctccttcacctgtgcatttgaattggtggccgttgacc gaaccttccatgaccaccttcatacgcatattttccttgatcagctCTTCGCCCTTAGACACCATggtgg ccattACAGCGTGGATGGCGTCTCcaggcgatctgacGGTTCACTAAACGAGCTCtttacgagggtagga agtggtacggaaagttggtataagacaaaagtgttgtggaattgctccaggcgatctgacggttcactaa acgagctctgcttttataggcgcccaccgtacacgcctaaagcttatacgttctctatcactgataggga gtaaactggatatacgttctctatcactgatagggagtaaactgtagatacgttctctatcactgatagg gagtaaactggtcatacgttctctatcactgatagggagtaaactccttatacgttctctatcactgata gggagtaaagtctgcatacgttctctatcactgatagggagtaaactcttcatacgttctctatcactga tagggagtaaactcgaggaatAGTACGAAcatggTAGctagtggatcCTTCGTCGACCTGCAGACTGGCT GTGTATAAGGGAGCCTGACATTTATATTCCCCAGAACATCAGGTTAATGGCGTTTTTGATGTCATTTTCG CGGTGGCTGAGATCAGCCACTTCTTCCCCGATAACGGAGACCGGCACACTGGCGCATATCGGTGGTCATC ATGCGCCAGCTTTCATCCCCGATATGCAcCACCGGGTAAAGTTCACGcatatgAAGCTGGACATCACCTC CCACAACCTCGAGGTGgaacagtacgctgatatctCTTATACTgggcagagcgcacatcgCCCACAGTCC CCGAGAAGTTGgGGGGAGGGGTCGGCAATTGAACCGGTGCCTAGAGAAGGTGGCGCGGGGTAAACTGGGA AAGTGATGTCGTGTACTGGCTCCGCCTTTTTCCCGAGGGTGGGGGAGAACCGTATATAAGTGCAGTAGTC GCCGTGAACGTTCTTTTTCGCAACGGgtttgccgccagaacacagTaatgtgccaccATGAGCcggctgg acaagagcaaagtgatcaacagcgccctggaactgctgaacggcgtgggcatcgagggcctgaccacccg gaagctggcccagaaactgggcgtggaacagcccaccctgtactggcacgtgaagaacaagcgggccctg ctggacgccctgcccatcgagatgctggaccggcaccacacccacagctgccctctggaaggcgagagct ggcaggacttcctgcggaacaacgccaagagctacagatgcgccctgctgagccaccgggacggcgccaa agtgcacctgggcaccagacccaccgagaagcagtacgagacactggaaaaccagctggccttcctgtgc cagcagggcttcagcctggaaaacgccctgtacgccctgagcgccgtgggccacttcaccctgggctgcg tgctggaagaacaggaacaccaggtcgccaaagaggaaagagagacacccaccaccgacagcatgccccc cctgctgaagcaggccatcgagctgttcgacagacagggcgccgagcccgccttcctgttcggcctggaa ctgatcatctgcggcctcgagaagcagctgaagtgcgagagcggcggacccaccgacgccctggacgact tcgacctggacatgctgcccgccgatgccctggatgattttgatctggatatgctccctgctgacgctct cgatgacttcgatctcgatatgctgccaggcggaaGCGGAGAAGGTAGAGgttctctcCTCACTTGTGGT GATGTTGAAGAAAACCCTGGtccaATGGTGAGCAAGGGCGAGgagctgttcaccggggtggtgcccatcc tggtcgagctggacggcgacgtaaacggccacaagttcagcgtccgcggcgagggcgagggcgatgccac caacggcaagctgaccctgaagttcatctgcaccaccggcaagctgcccgtgccctggcccaccctcgtg accaccttaggctacggcgtggcctgcttcgcccgctaccccgaccacatgaagcagcacgacttcttca agtccgccatgcccgaaggctacgtccaggagcgcaccatctctttcaaggacgacggtacctacaagac ccgcgccgaggtgaagttcgagggcgacaccctggtgaaccgcatcgtgctgaagggcatcgacttcaag gaggacggcaacatcctggggcacaagctggagtacaacttcaacagccacaaggtctatatcacggccg acaagcagaagaacggcatcaaggctaacttcaagacccgccacaacgttgaggacggcggcgtgcagct cgccgaccactaccagcagaacacccccatcggcgacggccccgtgctgctgcccgacaaccactacctg agccatcagtccaaactgagcaaagaccccaacgagaagcgcgatcacatggtcctgaaggagagggtga ccgccgccgggattacacatGACATGGACGAGCTGTACAAGggaagcggagctactaacttcagcctgct gaagcaggctggagacgtggaggagaaccctggacctATGACCGAGTACAAGCCCACGGtgcgcctcgcc acccgcgacgacgtccccagggccgtacgcaccctcgccgccgcgttcgccgactaccccgccacgcgcc acaccgtcgatccggaccgccacatcgagcgggtcaccgagctgcaagaactcttcctcacgcgcgtcgg gctcgacatcggcaaggtgtgggtcgcggacgacggcgccgcggtggcggtctggaccacgccggagagc gtcgaagcgggggcggtgttcgccgagatcggcccgcgcatggccgagttgagcggttcccggctggccg cgcagcaacagatggaaggcctcctggcgccgcaccggcccaaggagcccgcgtggttcctggccaccgt cggcgtctcgcccgaccaccagggcaagggtctgggcagcgccgtcgtgctccccggagtggaggcggcc gagcgcgccgggGTGCCCGCCTTCCTGGAGACaTCCGCGCCCCGCAACctccccttctacgagcggctcg gcttcaccgtcaccgccgacgtcgaggtgcccgaaggaccgcgcacctggtgcatgaccCGCAAGCCCGG TGCCTTGTAAcaacGCCGATAATCAACCTCTGGATTACAAAATTTGTGaaagattgactggtattcttaa ctatgttgctccttttacgctatgtggatacgctgctttaatgcctttgtatcatgctattgcttcccgt atggctttcattttctcctccttgtataaatcctggttgctgtctctttatgaggagttgtggcccgttg tcaggcaacgtggcgtggtgtgcactgtgtttgctgacgcaacccccactggttggggcattgccaccac ctgtcagctcctttccgggactttcgctttccccctccctattgccacggcggaactcatcgccgcctgc cttgcccgctgctggacaggggctcggctgttgggcactgacaattccgtggtgttgtcggggaaatcat cgtcctttccttggctgctcgcctgtgttgccacctggattctgcgcgggacgtccttctgctacgtccc ttcggccctcaatccagcggaccttccttcccgcggcctgctgccggctctgcggcctcttccgcgtctt cgccttcgccctcagacgagtcggatctcccTTTGGGCCGCCTCCCCGCCGCT

Partial Sequences from Depositor (2)

Sequence provided by depositing laboratory may be theoretical/predicted or based on Sanger/NGS sequencing results. Discrepancies between sequencing results obtained by Addgene and the original sequence provided by the depositor may be present.

Analyze Sequence

GenBank

SnapGene

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> mRuby2 ATGGTGTCTAAGGGCGAAGagctgatcaaggaaaatatgcgtatgaaggtggtcatggaaggttcggtca acggccaccaattcaaatgcacaggtgaaggagaaggcaatccgtacatgggaactcaaaccatgaggat caaagtcatcgagggaggacccctgccatttgcctttgacattcttgccacgtcgttcatgtatggcagc cgtacttttatcaagtacccgaaaggcattcctgatttctttaaacagtcctttcctgagggttttactt gggaaagagttacgagatacgaagatggtggagtcgtcaccgtcatgcaggacaccagccttgaggatgg ctgtctcgtttaccacgtccaagtcagaggggtaaactttccctccaatggtcccgtgatgcagaagaag accaagggttgggagcctaatacagagatgatgtatccagcagatggtggtctgaggggatacactcata tggcactgaaagttgatggtggtggccatctgtcttgctctttcgtaacaacttacaggtcaaaaaagac cgtcgggaacatcaagatgcccggtatccatgccgttgatcaccgcctggaaaggttagaggaaagtgac aatgaaatgttcgtagtacaacgcgaacacgcagttgccaagttcgccgggcttggtggtgggatGGACG AGCTGTACAAG
Analyze Sequence

GenBank

SnapGene

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> rtTA ATGAGCcggctggacaagagcaaagtgatcaacagcgccctggaactgctgaacggcgtgggcatcgagg gcctgaccacccggaagctggcccagaaactgggcgtggaacagcccaccctgtactggcacgtgaagaa caagcgggccctgctggacgccctgcccatcgagatgctggaccggcaccacacccacagctgccctctg gaaggcgagagctggcaggacttcctgcggaacaacgccaagagctacagatgcgccctgctgagccacc gggacggcgccaaagtgcacctgggcaccagacccaccgagaagcagtacgagacactggaaaaccagct ggccttcctgtgccagcagggcttcagcctggaaaacgccctgtacgccctgagcgccgtgggccacttc accctgggctgcgtgctggaagaacaggaacaccaggtcgccaaagaggaaagagagacacccaccaccg acagcatgccccccctgctgaagcaggccatcgagctgttcgacagacagggcgccgagcccgccttcct gttcggcctggaactgatcatctgcggcctcgagaagcagctgaagtgcgagagcggcggacccaccgac gccctggacgacttcgacctggacatgctgcccgccgatgccctggatgattttgatctggatatgctcc ctgctgacgctctcgatgacttcgatctcgatatgctgccaggc

pLentiX1-[TRE3G-mRuby2-TS-FF4x1-bGH]-EFS-rtTA-T2A-mGL-P2A-PuroR-WPRE Sequences (3)

Full Sequences from Addgene (1)

Partial Sequences from Depositor (2)

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