STK078_pSTK-0D-L3S3P21b
(Plasmid
#235413)
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PurposeVery strong terminator in B. subtilis. Strong synthetic transcriptional terminator in E. coli. Recombinant resistant. Modified to remove BsaI site (Position 49G to 49T).
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Depositing Lab
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Publication
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Sequence Information
Ordering
This material is available to academics and nonprofits only.
| Item | Catalog # | Description | Quantity | Price (USD) | |
|---|---|---|---|---|---|
| Plasmid | 235413 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $89 | |
Backbone
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Vector backbonepSB1A2
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Backbone manufactureriGEM
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Vector typeBacterial Expression
Growth in Bacteria
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Bacterial Resistance(s)Chloramphenicol, 25 μg/mL
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Growth Temperature37°C
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Growth Strain(s)DH5alpha
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Copy numberHigh Copy
Gene/Insert
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Gene/Insert nameL3S3P21
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SpeciesOther
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MutationNo
Cloning Information
- Cloning method Restriction Enzyme
- 5′ cloning site Unknown (unknown if destroyed)
Resource Information
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Supplemental Documents
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
How to cite this plasmid
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These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
STK078_pSTK-0D-L3S3P21b was a gift from Tom Ellis (Addgene plasmid # 235413 ; http://n2t.net/addgene:235413 ; RRID:Addgene_235413)
