STK080_pSTK-0D-bkdB Cold double terminator
(Plasmid
#235414)
-
PurposeVery strong terminator in B. subtilis. Transcriptional terminator and 3'-mRNA stabilizer from bkdB gene of B. subtilis. Positive impact in expression at 18C. Contain STOP codons in both directions
-
Depositing Lab
-
Publication
-
Sequence Information
Ordering
| Item | Catalog # | Description | Quantity | Price (USD) | |
|---|---|---|---|---|---|
| Plasmid | 235414 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $89 | |
Backbone
-
Vector backbonepSB1A2
-
Backbone manufactureriGEM
-
Vector typeBacterial Expression
Growth in Bacteria
-
Bacterial Resistance(s)Chloramphenicol, 25 μg/mL
-
Growth Temperature37°C
-
Growth Strain(s)DH5alpha
-
Copy numberHigh Copy
Gene/Insert
-
Gene/Insert namebkdB Cold double terminator
-
SpeciesOther
-
MutationNo
Cloning Information
- Cloning method Restriction Enzyme
- 5′ cloning site Unknown (unknown if destroyed)
Resource Information
-
Supplemental Documents
Terms and Licenses
-
Academic/Nonprofit Terms
-
Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
-
For your Materials & Methods section:
STK080_pSTK-0D-bkdB Cold double terminator was a gift from Tom Ellis (Addgene plasmid # 235414 ; http://n2t.net/addgene:235414 ; RRID:Addgene_235414)