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Addgene

pHK302.4
(Plasmid #235469)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 235469 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    pBR322/pET
  • Vector type
    Synthetic Biology

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    sgRNA4
  • gRNA/shRNA sequence
    GACACATCTTAGAGTATGTAGTTTcAGAGCTATGCTGGAAACAGCATAGCAAGTTgAAATAAGGCTAGTCCGTTATCAACTTGAAAAAGTGGCACCGAGTCGGTGC
  • Species
    Synthetic

Cloning Information

  • Cloning method Gibson Cloning

Resource Information

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.

Depositor Comments

JM101 (working strain for phagemid transfer). Requires the helper phagemid, e.g. HP17_KO7 (Addgene plasmid #120346). Please visit https://doi.org/10.1101/2024.08.28.610043 for bioRxiv preprint.

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pHK302.4 was a gift from Yolanda Schaerli (Addgene plasmid # 235469 ; http://n2t.net/addgene:235469 ; RRID:Addgene_235469)

  • For your References section:

    Engineering intercellular communication using M13 phagemid and CRISPR-based gene regulation for multicellular computing in Escherichia coli. Kusumawardhani, H., Zoppi, F., Avendaño, R. et al.. Nat Commun 16, 3569 (2025) 10.1038/s41467-025-58760-z
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