pEGFP-TDP-43 (K136R)
(Plasmid
#235492)
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PurposeExpression of human TDP-43-EGFP with K136R mutation
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Depositing Lab
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Sequence Information
Ordering
Item | Catalog # | Description | Quantity | Price (USD) | |
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Plasmid | 235492 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 * |
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Backbone
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Vector backbonepEGFP-N3
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Vector typeMammalian Expression
Growth in Bacteria
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Bacterial Resistance(s)Kanamycin, 50 μg/mL
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Growth Temperature37°C
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Growth Strain(s)DH5alpha
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Copy numberHigh Copy
Gene/Insert
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Gene/Insert nameTARDBP
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Alt nameTDP-43
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SpeciesH. sapiens (human)
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MutationK136R
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Entrez GeneTARDBP (a.k.a. ALS10, TDP-43)
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Tag
/ Fusion Protein
- EGFP (C terminal on backbone)
Cloning Information
- Cloning method Restriction Enzyme
- 5′ cloning site Unknown (unknown if destroyed)
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
Trademarks:
- Zeocin® is an InvivoGen trademark.
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
pEGFP-TDP-43 (K136R) was a gift from Maxime Rousseaux (Addgene plasmid # 235492 ; http://n2t.net/addgene:235492 ; RRID:Addgene_235492) -
For your References section:
A stress-dependent TDP-43 SUMOylation program preserves neuronal function. Suk TR, Part CE, Zhang JL, Nguyen TT, Heer MM, Caballero-Gomez A, Grybas VS, McKeever PM, Nguyen B, Ali T, Callaghan SM, Woulfe JM, Robertson J, Rousseaux MWC. Mol Neurodegener. 2025 Mar 28;20(1):38. doi: 10.1186/s13024-025-00826-z. 10.1186/s13024-025-00826-z PubMed 40149017