pChd4 GGSG_mAID-3xF_BactBsd
              
              
                (Plasmid
                
                #235508)
              
            
            
            
          - 
            PurposeTargeting vector for Chd4-miniAID fusion, Roxed Bact-Bsd
 - 
              Depositing Lab
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          Publication
 - 
          Sequence Information
 
Ordering
| Item | Catalog # | Description | Quantity | Price (USD) | |
|---|---|---|---|---|---|
| Plasmid | 235508 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $89 | |
Backbone
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            Vector backbonepBluescript II KS-
 - Backbone size w/o insert (bp) 3000
 - 
              Vector typeMouse Targeting
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                Selectable markersBlasticidin
 
Growth in Bacteria
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            Bacterial Resistance(s)Ampicillin, 100 μg/mL
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            Growth Temperature37°C
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            Growth Strain(s)DH5alpha
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            Copy numberHigh Copy
 
Gene/Insert
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                Gene/Insert nameChd4
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                    SpeciesM. musculus (mouse)
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                  MutationNone
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                        Entrez GeneChd4 (a.k.a. 9530019N15Rik, D6Ertd380e, Mi-2beta, mKIAA4075)
 - Promoter beta actin
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        Tag
        / Fusion Protein
    
- mAID, 3xFLAG (C terminal on insert)
 
 
Cloning Information
- Cloning method Gibson Cloning
 
Resource Information
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            Supplemental Documents
 
Terms and Licenses
- 
        Academic/Nonprofit Terms
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      Industry Terms
- Not Available to Industry
 
 
Trademarks:
- Zeocin® is an InvivoGen trademark.
 
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
pChd4 GGSG_mAID-3xF_BactBsd was a gift from Brian Hendrich (Addgene plasmid # 235508 ; http://n2t.net/addgene:235508 ; RRID:Addgene_235508)