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pTRIP-SFFV-EGFP-FLAG-UL36USP (C65)
(Plasmid #235549)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 235549 Standard format: Plasmid sent in bacteria as agar stab 1 $89
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Backbone

  • Vector backbone
    pTRIP-SFFV
  • Vector type
    Lentiviral

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    30°C
  • Growth Strain(s)
    NEB Stable
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    UL36USP (C65)
  • Species
    Herpes simplex virus 1 (HSV-1)
  • Mutation
    N-terminal part of UL36 starting at the first ATG of UL36 (leading to a cysteine at position 65). Amino acids 1-520.
  • Tag / Fusion Protein
    • EGFP-FLAG (N terminal on insert)

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site Unknown (unknown if destroyed)

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pTRIP-SFFV-EGFP-FLAG-UL36USP (C65) was a gift from Nicolas Manel (Addgene plasmid # 235549 ; http://n2t.net/addgene:235549 ; RRID:Addgene_235549)

  • For your References section:

    Centromeric DNA amplification triggered by viral proteins activates nuclear cGAS. Lahaye X, Tran Van P, Chakraborty C, Shmakova A, Cao NTB, Ferran H, Ait-Mohamed O, Maurin M, Waterfall JJ, Kaufer BB, Fischer P, Hennig T, Dolken L, Lomonte P, Fachinetti D, Manel N. Cell. 2025 May 29:S0092-8674(25)00556-2. doi: 10.1016/j.cell.2025.05.008. 10.1016/j.cell.2025.05.008 PubMed 40460826
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