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pPB_TRE3G_scFV-sfGFP-G9aCD
(Plasmid #235580)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 235580 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    pPB-TRE3G
  • Vector type
    CRISPR

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    30°C
  • Growth Strain(s)
    NEB Stable
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    G9a
  • Alt name
    Ehmt2
  • Species
    M. musculus (mouse)
  • Entrez Gene
    Ehmt2 (a.k.a. Bat8, D17Ertd710e, G9a, KMT1C, NG36)

Cloning Information

  • Cloning method Gibson Cloning

Resource Information

Terms and Licenses

Trademarks:
  • Zeocin® is an InvivoGen trademark.

Depositor Comments

This is a piggyBAC plasmid that integrates into the genome and can be selected for via Neomycin resistance. Expression of epigenetic effector is induced via adding doxycyline to media. Succesful activiation of the epigenetic editing system is indicated by sfGFP expression.

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pPB_TRE3G_scFV-sfGFP-G9aCD was a gift from Jamie Hackett (Addgene plasmid # 235580 ; http://n2t.net/addgene:235580 ; RRID:Addgene_235580)

  • For your References section:

    Systematic epigenome editing captures the context-dependent instructive function of chromatin modifications. Policarpi C, Munafo M, Tsagkris S, Carlini V, Hackett JA. Nat Genet. 2024 Jun;56(6):1168-1180. doi: 10.1038/s41588-024-01706-w. Epub 2024 May 9. 10.1038/s41588-024-01706-w PubMed 38724747
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