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EcoHIV-mScarlet
(Plasmid #235598)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 235598 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    pUC18
  • Backbone size w/o insert (bp) 2686
  • Total vector size (bp) 16667
  • Vector type
    Lentiviral

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    NEB Stable
  • Copy number
    Low Copy

Gene/Insert

  • Gene/Insert name
    NL4-3 EcoHIV mScarlet
  • Alt name
    NL-4-3 HIV
  • Alt name
    mScarlet
  • Alt name
    MLV gp80
  • Species
    Synthetic
  • GenBank ID
    AF324493.2 GCF_000864765.1
  • Tag / Fusion Protein
    • mScarlet

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site Cla I (not destroyed)
  • 3′ cloning site Not I (not destroyed)
  • 5′ sequencing primer ND
    • (Common Sequencing Primers)

Resource Information

  • Supplemental Documents
  • A portion of this plasmid was derived from a plasmid made by
    David Volsky of Mount Sinai. Modification of plasmid pNL4-3-EcoHIV (Potash et al., 2005; PMID: 15728729).

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.

Depositor Comments

The construct is a modification of pNL4-3-EcoHIV ( M.J. Potash, W. Chao, G. Bentsman, N. Paris, M. Saini, J. Nitkiewicz, P. Belem, L. Sharer, A.I. Brooks, & D.J. Volsky, A mouse model for study of systemic HIV-1 infection, antiviral immune responses, and neuroinvasiveness, Proc. Natl. Acad. Sci. U.S.A. 102 (10) 3760-3765, https://doi.org/10.1073/pnas.0500649102 (2005).) with inserted mScarlet.

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    EcoHIV-mScarlet was a gift from Michael Shtutman (Addgene plasmid # 235598 ; http://n2t.net/addgene:235598 ; RRID:Addgene_235598)

  • For your References section:

    Identification of EcoHIV-Infected Cells in Microglia-Manipulated Transgenic Mice. Li H, Walker M, Ji H, Sikirzhytskaya A, Aksenova M, Shtutman M, Sikirzhytski V, Mactutus CF, Booze RM. J Vis Exp. 2024 Dec 20;(214). doi: 10.3791/67150. 10.3791/67150 PubMed 39760359
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