pOPINK-CAPN15 ZF4 (337-369)
(Plasmid
#235648)
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PurposeBacterial expression of human CAPN15 ZF4 (337-369)
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Depositing Lab
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Sequence Information
Ordering
Item | Catalog # | Description | Quantity | Price (USD) | |
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Plasmid | 235648 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 |
Backbone
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Vector backbonepOPIN-K
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Vector typeBacterial Expression
Growth in Bacteria
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Bacterial Resistance(s)Kanamycin, 50 μg/mL
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Growth Temperature37°C
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Growth Strain(s)DH5alpha
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Copy numberLow Copy
Gene/Insert
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Gene/Insert nameCAPN15 ZF4
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SpeciesH. sapiens (human)
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Mutationaa 337-369 only
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Entrez GeneCAPN15 (a.k.a. SOLH)
- Promoter T7
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Tag
/ Fusion Protein
- 6xHis-GST-3c (N terminal on backbone)
Cloning Information
- Cloning method Other
Resource Information
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Supplemental Documents
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
pOPINK-CAPN15 ZF4 (337-369) was a gift from David Komander (Addgene plasmid # 235648 ; http://n2t.net/addgene:235648 ; RRID:Addgene_235648) -
For your References section:
Secondary interactions in ubiquitin-binding domains achieve linkage or substrate specificity. Michel MA, Scutts S, Komander D. Cell Rep. 2024 Aug 27;43(8):114545. doi: 10.1016/j.celrep.2024.114545. Epub 2024 Jul 23. 10.1016/j.celrep.2024.114545 PubMed 39052481