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Addgene

pOPINK-Sharpin NZF (347-376)
(Plasmid #235651)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 235651 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    pOPIN-K
  • Vector type
    Bacterial Expression

Growth in Bacteria

  • Bacterial Resistance(s)
    Kanamycin, 50 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    Low Copy

Gene/Insert

  • Gene/Insert name
    Sharpin NZF
  • Species
    H. sapiens (human)
  • Mutation
    aa 347-376 only
  • Entrez Gene
    SHARPIN (a.k.a. SIPL1)
  • Promoter T7
  • Tag / Fusion Protein
    • 6xHis-GST-3c (N terminal on backbone)

Cloning Information

  • Cloning method Other

Resource Information

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pOPINK-Sharpin NZF (347-376) was a gift from David Komander (Addgene plasmid # 235651 ; http://n2t.net/addgene:235651 ; RRID:Addgene_235651)

  • For your References section:

    Secondary interactions in ubiquitin-binding domains achieve linkage or substrate specificity. Michel MA, Scutts S, Komander D. Cell Rep. 2024 Aug 27;43(8):114545. doi: 10.1016/j.celrep.2024.114545. Epub 2024 Jul 23. 10.1016/j.celrep.2024.114545 PubMed 39052481
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