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Addgene

pcDNA3.1(+)-Flag-SOD1-G86R
(Plasmid #235663)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 235663 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    pcDNA3.1(+)
  • Vector type
    Mammalian Expression
  • Selectable markers
    Neomycin (select with G418)

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    SOD1-G86R
  • Species
    H. sapiens (human)
  • Mutation
    changed Glycine 86 to arginine
  • Entrez Gene
    SOD1 (a.k.a. ALS, ALS1, HEL-S-44, IPOA, SOD, STAHP, hSod1, homodimer)
  • Promoter CMV promoter
  • Tag / Fusion Protein
    • Flag-tag at N-terminal

Cloning Information

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pcDNA3.1(+)-Flag-SOD1-G86R was a gift from Chen Seng Ng (Addgene plasmid # 235663 ; http://n2t.net/addgene:235663 ; RRID:Addgene_235663)

  • For your References section:

    Integrated transcriptomic profiling reveals a STING-mediated Type II Interferon signature in SOD1-mutant amyotrophic lateral sclerosis models. Hiew JY, Lim YS, Liu H, Ng CS. Commun Biol. 2025 Mar 2;8(1):347. doi: 10.1038/s42003-025-07790-w. 10.1038/s42003-025-07790-w PubMed 40025162
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