pYO1101
(Plasmid #235708)

• Purpose: Expression of FL human Beclin 1 and FL human ATG14L in mammalian cells
• Depositing Lab: Roger Williams
• Publication: Ohashi et al Elife. 2020 Jun 30;9:e58281. doi: 10.7554/eLife.58281.

Backbone

• Vector backbone: pCAG
• Vector type: Mammalian Expression

Growth in Bacteria

• Bacterial Resistance(s): Ampicillin, 100 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): DH5alpha
• Growth instructions: Depositing lab also recommends XL10-gold E. coli for transformation.
• Copy number: High Copy

Gene/Insert 1

• Gene/Insert name: Beclin 1
• Species: H. sapiens (human)
• Entrez Gene: BECN1 (a.k.a. ATG6, VPS30, beclin1)
• Promoter: CMV

Cloning Information for Gene/Insert 1

• Cloning method: Gibson Cloning
• 5′ sequencing primer: n/a

Gene/Insert 2

• Gene/Insert name: ATG14L
• Species: H. sapiens (human)
• Entrez Gene: ATG14 (a.k.a. ATG14L, BARKOR, KIAA0831)
• Promoter: CAG

Cloning Information for Gene/Insert 2

• Cloning method: Gibson Cloning
• 5′ sequencing primer: n/a

Resource Information

• Supplemental Documents:
  • pYO1101.gb
• Article Citing this Plasmid:
  • 1 Reference

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
• Industry Terms:
  • Not Available to Industry

Depositor Comments

For other work using this plasmid, please see Tremel et al. 2021 PMID 33692360.