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Addgene

pYO1296
(Plasmid #235710)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 235710 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    pEGFP-C1
  • Vector type
    Mammalian Expression

Growth in Bacteria

  • Bacterial Resistance(s)
    Kanamycin, 50 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    RAB5
  • Species
    H. sapiens (human)
  • Mutation
    Q79L, aa 2-215
  • Entrez Gene
    RAB5A (a.k.a. RAB5)
  • Tag / Fusion Protein
    • mCherry (N terminal on insert)

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site Unknown (unknown if destroyed)

Resource Information

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pYO1296 was a gift from Roger Williams (Addgene plasmid # 235710 ; http://n2t.net/addgene:235710 ; RRID:Addgene_235710)

  • For your References section:

    Structural basis for VPS34 kinase activation by Rab1 and Rab5 on membranes. Tremel S, Ohashi Y, Morado DR, Bertram J, Perisic O, Brandt LTL, von Wrisberg MK, Chen ZA, Maslen SL, Kovtun O, Skehel M, Rappsilber J, Lang K, Munro S, Briggs JAG, Williams RL. Nat Commun. 2021 Mar 10;12(1):1564. doi: 10.1038/s41467-021-21695-2. 10.1038/s41467-021-21695-2 PubMed 33692360
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