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Addgene

pOP823
(Plasmid #235718)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 235718 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    pOPINS
  • Vector type
    Bacterial Expression

Growth in Bacteria

  • Bacterial Resistance(s)
    Kanamycin, 50 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    RAB5A
  • Species
    H. sapiens (human)
  • Mutation
    Q79L, C19S, C63S, aa 1-212
  • Entrez Gene
    RAB5A (a.k.a. RAB5)
  • Tag / Fusion Protein
    • His6-SUMO (N terminal on backbone)

Cloning Information

  • Cloning method Ligation Independent Cloning

Resource Information

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.

Depositor Comments

For other work using this plasmid, please see Tremel et al. 2021 PMID 33692360.

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pOP823 was a gift from Roger Williams (Addgene plasmid # 235718 ; http://n2t.net/addgene:235718 ; RRID:Addgene_235718)

  • For your References section:

    The G-Protein Rab5A Activates VPS34 Complex II, a Class III PI3K, by a Dual Regulatory Mechanism. Buckles TC, Ohashi Y, Tremel S, McLaughlin SH, Pardon E, Steyaert J, Gordon MT, Williams RL, Falke JJ. Biophys J. 2020 Dec 1;119(11):2205-2218. doi: 10.1016/j.bpj.2020.10.028. Epub 2020 Oct 31. 10.1016/j.bpj.2020.10.028 PubMed 33137306
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