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pYO395
(Plasmid #235734)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 235734 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    pcDNA4.0/TO
  • Vector type
    Mammalian Expression

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Growth instructions
    Depositing lab also recommends XL10-gold E. coli for transformation.
  • Copy number
    High Copy

Gene/Insert 1

  • Gene/Insert name
    ATG14
  • Species
    H. sapiens (human)
  • Mutation
    N256S
  • Entrez Gene
    ATG14 (a.k.a. ATG14L, BARKOR, KIAA0831)
  • Tag / Fusion Protein
    • ZZ-3xTEV (N terminal on insert)

Cloning Information for Gene/Insert 1

Gene/Insert 2

  • Gene/Insert name
    EGFP
  • Promoter CMV

Cloning Information for Gene/Insert 2

Gene/Insert 3

  • Gene/Insert name
    BECN1
  • Species
    H. sapiens (human)
  • Entrez Gene
    BECN1 (a.k.a. ATG6, VPS30, beclin1)
  • Promoter CMV

Cloning Information for Gene/Insert 3

Gene/Insert 4

  • Gene/Insert name
    PIK3C3
  • Species
    H. sapiens (human)
  • Entrez Gene
    PIK3C3 (a.k.a. VPS34, Vps34, hVps34)
  • Promoter CMV

Cloning Information for Gene/Insert 4

Gene/Insert 5

  • Gene/Insert name
    PIK3R4
  • Species
    H. sapiens (human)
  • Entrez Gene
    PIK3R4 (a.k.a. VPS15, p150)
  • Promoter CMV

Cloning Information for Gene/Insert 5

Resource Information

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.

Depositor Comments

For other work using this plasmid, please see Mercer et al. 2021 PMID 34121209.

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pYO395 was a gift from Roger Williams (Addgene plasmid # 235734 ; http://n2t.net/addgene:235734 ; RRID:Addgene_235734)

  • For your References section:

    Characterization of Atg38 and NRBF2, a fifth subunit of the autophagic Vps34/PIK3C3 complex. Ohashi Y, Soler N, Garcia Ortegon M, Zhang L, Kirsten ML, Perisic O, Masson GR, Burke JE, Jakobi AJ, Apostolakis AA, Johnson CM, Ohashi M, Ktistakis NT, Sachse C, Williams RL. Autophagy. 2016 Nov;12(11):2129-2144. doi: 10.1080/15548627.2016.1226736. Epub 2016 Sep 14. 10.1080/15548627.2016.1226736 PubMed 27630019
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