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Addgene

pMO14
(Plasmid #235736)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 235736 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    pcDNA4.0/TO
  • Vector type
    Mammalian Expression

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Growth instructions
    Depositing lab also recommends XL10-gold E. coli for transformation.
  • Copy number
    High Copy

Gene/Insert 1

  • Gene/Insert name
    ATG14L
  • Species
    H. sapiens (human)
  • Mutation
    N256S
  • Entrez Gene
    ATG14 (a.k.a. ATG14L, BARKOR, KIAA0831)
  • Promoter CMV
  • Tag / Fusion Protein
    • ZZ-3xTEV (N terminal on insert)

Cloning Information for Gene/Insert 1

Gene/Insert 2

  • Gene/Insert name
    UVRAG
  • Species
    H. sapiens (human)
  • Entrez Gene
    UVRAG (a.k.a. DHTX, VPS38, p63)
  • Promoter CMV
  • Tag / Fusion Protein
    • 2xStrep-tagII-2xTEV-ZZ (C terminal on insert)

Cloning Information for Gene/Insert 2

Gene/Insert 3

  • Gene/Insert name
    EGFP
  • Promoter CMV

Cloning Information for Gene/Insert 3

Gene/Insert 4

  • Gene/Insert name
    BECN1
  • Species
    H. sapiens (human)
  • Entrez Gene
    BECN1 (a.k.a. ATG6, VPS30, beclin1)
  • Promoter CMV

Cloning Information for Gene/Insert 4

Gene/Insert 5

  • Gene/Insert name
    PIK3C3
  • Species
    H. sapiens (human)
  • Entrez Gene
    PIK3C3 (a.k.a. VPS34, Vps34, hVps34)
  • Promoter CMV

Cloning Information for Gene/Insert 5

Gene/Insert 6

  • Gene/Insert name
    PIK3R4
  • Species
    H. sapiens (human)
  • Entrez Gene
    PIK3R4 (a.k.a. VPS15, p150)
  • Promoter CMV

Cloning Information for Gene/Insert 6

Resource Information

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pMO14 was a gift from Roger Williams (Addgene plasmid # 235736 ; http://n2t.net/addgene:235736 ; RRID:Addgene_235736)

  • For your References section:

    Phosphoproteomic identification of ULK substrates reveals VPS15-dependent ULK/VPS34 interplay in the regulation of autophagy. Mercer TJ, Ohashi Y, Boeing S, Jefferies HBJ, De Tito S, Flynn H, Tremel S, Zhang W, Wirth M, Frith D, Snijders AP, Williams RL, Tooze SA. EMBO J. 2021 Jul 15;40(14):e105985. doi: 10.15252/embj.2020105985. Epub 2021 Jun 14. 10.15252/embj.2020105985 PubMed 34121209
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