pYO524
(Plasmid
#235744)
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PurposeProtein expression of KmLst8 by integrating KmLST8 into the K. marxianus genome
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Depositing Lab
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Sequence Information
Ordering
Item | Catalog # | Description | Quantity | Price (USD) | |
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Plasmid | 235744 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 |
Backbone
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Vector backbonepBluescuript II (KS-)
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Vector typeYeast Expression
Growth in Bacteria
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Bacterial Resistance(s)Ampicillin, 100 μg/mL
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Growth Temperature37°C
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Growth Strain(s)DH5alpha
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Copy numberHigh Copy
Gene/Insert
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Gene/Insert nameKmLST8
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SpeciesK. marxianus
Cloning Information
- Cloning method Restriction Enzyme
- 5′ cloning site Unknown (unknown if destroyed)
Resource Information
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Supplemental Documents
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
pYO524 was a gift from Roger Williams (Addgene plasmid # 235744 ; http://n2t.net/addgene:235744 ; RRID:Addgene_235744) -
For your References section:
Tor forms a dimer through an N-terminal helical solenoid with a complex topology. Baretic D, Berndt A, Ohashi Y, Johnson CM, Williams RL. Nat Commun. 2016 Apr 13;7:11016. doi: 10.1038/ncomms11016. 10.1038/ncomms11016 PubMed 27072897