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pYO391
(Plasmid #235751)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 235751 Standard format: Plasmid sent in bacteria as agar stab 1 $89
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Backbone

  • Vector backbone
    pRS426
  • Vector type
    Yeast Expression

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    XL10 Gold
  • Growth instructions
    Depositing lab also recommends XL10-gold E. coli for transformation.
  • Copy number
    High Copy

Gene/Insert 1

  • Gene/Insert name
    VPS34
  • Species
    S. cerevisiae (budding yeast)
  • Mutation
    S2A
  • Entrez Gene
    VPS34 (a.k.a. YLR240W, END12, PEP15, STT8, VPL7, VPS7, VPT29)

Cloning Information for Gene/Insert 1

  • Cloning method Restriction Enzyme
  • 5′ cloning site Unknown (unknown if destroyed)
  • 3′ cloning site Unknown (unknown if destroyed)
  • 5′ sequencing primer n/a
    • (Common Sequencing Primers)

Gene/Insert 2

  • Gene/Insert name
    VPS15
  • Species
    S. cerevisiae (budding yeast)
  • Mutation
    T134A, I851R
  • Entrez Gene
    VPS15 (a.k.a. YBR097W, GRD8, VAC4, VPL19, VPS40, VPT15)
  • Promoter GALGAPDH
  • Tag / Fusion Protein
    • 3xTEV-ZZ (C terminal on insert)

Cloning Information for Gene/Insert 2

  • Cloning method Restriction Enzyme
  • 5′ cloning site Unknown (unknown if destroyed)
  • 3′ cloning site Unknown (unknown if destroyed)
  • 5′ sequencing primer n/a
    • (Common Sequencing Primers)

Gene/Insert 3

  • Gene/Insert name
    VPS38
  • Species
    S. cerevisiae (budding yeast)
  • Entrez Gene
    VPS38 (a.k.a. YLR360W, VPL17)

Cloning Information for Gene/Insert 3

  • Cloning method Restriction Enzyme
  • 5′ cloning site Unknown (unknown if destroyed)
  • 3′ cloning site Unknown (unknown if destroyed)
  • 5′ sequencing primer n/a
    • (Common Sequencing Primers)

Gene/Insert 4

  • Gene/Insert name
    VPS30
  • Species
    S. cerevisiae (budding yeast)
  • Entrez Gene
    VPS30 (a.k.a. YPL120W, APG6, ATG6, VPT30)
  • Tag / Fusion Protein
    • ZZ-3xTEV (N terminal on insert)

Cloning Information for Gene/Insert 4

  • Cloning method Restriction Enzyme
  • 5′ cloning site Unknown (unknown if destroyed)
  • 3′ cloning site Unknown (unknown if destroyed)
  • 5′ sequencing primer n/a
    • (Common Sequencing Primers)

Resource Information

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pYO391 was a gift from Roger Williams (Addgene plasmid # 235751 ; http://n2t.net/addgene:235751 ; RRID:Addgene_235751)

  • For your References section:

    Structure and flexibility of the endosomal Vps34 complex reveals the basis of its function on membranes. Rostislavleva K, Soler N, Ohashi Y, Zhang L, Pardon E, Burke JE, Masson GR, Johnson C, Steyaert J, Ktistakis NT, Williams RL. Science. 2015 Oct 9;350(6257):aac7365. doi: 10.1126/science.aac7365. 10.1126/science.aac7365 PubMed 26450213
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