pYO1920
(Plasmid
#235767)
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PurposeProtein expression of ScVPS34 untagged + ScVPS15 S622D_H623E_T626E-ZZ in yeast
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Depositing Lab
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Publication
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Sequence Information
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Please contact us if you would like Addgene to sequence a portion of this plasmid before you request it.
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Full plasmid sequence is not available for this item.
Ordering
| Item | Catalog # | Description | Quantity | Price (USD) | |
|---|---|---|---|---|---|
| Plasmid | 235767 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $89 | |
Backbone
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Vector backbonepRS424
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Vector typeYeast Expression
Growth in Bacteria
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Bacterial Resistance(s)Ampicillin, 100 μg/mL
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Growth Temperature37°C
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Growth Strain(s)DH5alpha
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Copy numberHigh Copy
Gene/Insert 1
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Gene/Insert nameVPS34
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SpeciesS. cerevisiae (budding yeast)
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MutationScVPS15 S622D_H623E_T626E
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Entrez GeneVPS34 (a.k.a. YLR240W, END12, PEP15, STT8, VPL7, VPS7, VPT29)
- Promoter GAL-TDH3
Cloning Information for Gene/Insert 1
- Cloning method Ligation Independent Cloning
- 5′ sequencing primer n/a
- (Common Sequencing Primers)
Gene/Insert 2
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Gene/Insert nameVPS15
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SpeciesS. cerevisiae (budding yeast)
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MutationS622D, H623E, T626E
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Entrez GeneVPS15 (a.k.a. YBR097W, GRD8, VAC4, VPL19, VPS40, VPT15)
- Promoter GAL-TDH3
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Tag
/ Fusion Protein
- 3xTEV-ZZ (C terminal on insert)
Cloning Information for Gene/Insert 2
- Cloning method Ligation Independent Cloning
- 5′ sequencing primer n/a
- (Common Sequencing Primers)
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
pYO1920 was a gift from Roger Williams (Addgene plasmid # 235767)