pYY62-P1int-TP901-GFPdropout
(Plasmid
#235773)
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Purpose(Empty Backbone) Plasmid used for constructing donor DNA for TP901-compatible p1 integration. This plasmid is a sfGFP dropout plasmid where sfGFP can be replaced by GOIs using Golden Gate Assembly.
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Depositing Lab
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Sequence Information
Ordering
This material is available to academics and nonprofits only.
| Item | Catalog # | Description | Quantity | Price (USD) | |
|---|---|---|---|---|---|
| Plasmid | 235773 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 | |
Backbone
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Vector backbonepMono_T
Growth in Bacteria
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Bacterial Resistance(s)Ampicillin, 100 μg/mL
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Growth Temperature37°C
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Growth Strain(s)DH5alpha
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Copy numberUnknown
Resource Information
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Supplemental Documents
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
Depositor Comments
Please visit https://doi.org/10.1101/2024.11.29.626108 for bioRxiv preprint.
How to cite this plasmid
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These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
pYY62-P1int-TP901-GFPdropout was a gift from Chang Liu (Addgene plasmid # 235773 ; http://n2t.net/addgene:235773 ; RRID:Addgene_235773) -
For your References section:
Ultra-Efficient Integration of Gene Libraries onto Yeast Cytosolic Plasmids. Pisera A, Yu Y, Williams RL, Liu CC. bioRxiv [Preprint]. 2024 Nov 30:2024.11.29.626108. doi: 10.1101/2024.11.29.626108. 10.1101/2024.11.29.626108 PubMed 39651169
Map uploaded by the depositor.
