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pME_Cp_0_7-8_003_Amp/ColEI_mScarlet-I
(Plasmid #235969)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 235969 Standard format: Plasmid sent in bacteria as agar stab 1 $89
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Backbone

  • Vector backbone
    pYTK083
  • Backbone size w/o insert (bp) 1867
  • Vector type
    Synthetic Biology

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    Amp/ColEI
  • Species
    Discosoma sp.
  • Insert Size (bp)
    1019

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site BsaI (destroyed during cloning)
  • 3′ cloning site BsaI (destroyed during cloning)
  • 5′ sequencing primer ACATGTTCTTTCCTGCGTTATC
  • 3′ sequencing primer GGTAACTGTCAGACCAAGTTTAC
    • (Common Sequencing Primers)

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.

Depositor Comments

Please visit https://doi.org/10.1101/2024.05.08.593163 for bioRxiv preprint.

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pME_Cp_0_7-8_003_Amp/ColEI_mScarlet-I was a gift from Tobias Erb (Addgene plasmid # 235969 ; http://n2t.net/addgene:235969 ; RRID:Addgene_235969)

  • For your References section:

    Advancing chloroplast synthetic biology through high-throughput plastome engineering of Chlamydomonas reinhardtii. Inckemann R, Chotel T, Brinkmann CK, Burgis M, Andreas L, Baumann J, Sharma P, Klose M, Barrett J, Ries F, Paczia N, Glatter T, Willmund F, Mackinder LCM, Erb TJ. bioRxiv 2024.05.08.593163 10.1101/2024.05.08.593163
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