pME_G_E_007_UAV_sfGFP_PaqCI
(Plasmid
#235975)
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PurposeUniversal acceptor vector with sfGFP placeholder PaqCI compatible for lvl0 assembly
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Depositing Lab
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Sequence Information
Ordering
Item | Catalog # | Description | Quantity | Price (USD) | |
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Plasmid | 235975 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 |
Backbone
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Vector backbonepSB1C3
- Backbone size w/o insert (bp) 2093
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Vector typeSynthetic Biology
Growth in Bacteria
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Bacterial Resistance(s)Chloramphenicol, 25 μg/mL
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Growth Temperature37°C
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Growth Strain(s)DH5alpha
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Copy numberHigh Copy
Gene/Insert
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Gene/Insert nameUAV_sfGFP_PaqCI
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SpeciesAequoria victoria
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Insert Size (bp)1042
Cloning Information
- Cloning method Restriction Enzyme
- 5′ cloning site PaqCI (destroyed during cloning)
- 3′ cloning site PaqCI (destroyed during cloning)
- 5′ sequencing primer CGTATCACGAGGCAGAATTTC
- 3′ sequencing primer CCTGCATAACGCGAAGTAATC (Common Sequencing Primers)
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
Depositor Comments
Please visit https://doi.org/10.1101/2024.05.08.593163 for bioRxiv preprint.
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
pME_G_E_007_UAV_sfGFP_PaqCI was a gift from Tobias Erb (Addgene plasmid # 235975 ; http://n2t.net/addgene:235975 ; RRID:Addgene_235975)